Journal: Cancer Management and Research

Article Title: Inhibition of CDK1 Promotes Immunogenic Cell Death in Neuroblastoma

doi: 10.2147/CMAR.S569214

Figure Lengend Snippet: Overexpression of CDK1 is associated with poor outcome of Neuroblastoma. ( A ) The expression of CDK1 was positively correlated with the stage of NB; ( B ) IHC confirmed that the expression of CDK1 was positively associated with the stage of NB; ( C ) CDK1 expression is correlated with MYCN amplification; ( D ) The expression of CDK1 showed a negative association with the prognosis of NB.

Article Snippet: A total of 5×10 5 SKNSH and SKNBE cells were cultured in 6-well plates and incubated with DMSO, 10 μM, 15 μM, 20 μM, or 25 μM of the CDK1 inhibitor Ro-3306 (MedChemExpress, HY-12529) for 24 h. Then, the cells were collected, resuspended in binding buffer and stained with Annexin V-FITC/PI (KeyGEN BioTECH, KGA1102) for 15 min at RT in the dark.

Techniques: Over Expression, Expressing, Amplification

Journal: Cancer Management and Research

Article Title: Inhibition of CDK1 Promotes Immunogenic Cell Death in Neuroblastoma

doi: 10.2147/CMAR.S569214

Figure Lengend Snippet: High expression of CDK1 correlated with lower immunogenicity and effector immune cells infiltration. ( A ) The expression of CDK1 was negatively associated with immune score in NB (CDK1-low (n=75); CDK1-high (n=75); ( B ) The expression of CDK1 showed a negative correlation with stromal score in NB (CDK1-low (n=75); CDK1-high (n=75)); ( C ) The expression of CDK1 was negatively related with estimate score in NB (CDK1-low (n=75); CDK1-high (n=75)); ( D ) The expression of CDK1 was positively associated with TumorPurity (CDK1-low (n=75); CDK1-high (n=75)). ( E ) The expression of CDK1 exhibited a negative association with the infiltration of activated DCs in NB; ( F ) The expression of CDK1 exhibited a negative association with the infiltration of activated CD8 + T cells in NB; ( G ) The expression of CDK1 exhibited a negative association with the infiltration of NK cells in NB; ( H ) The association between the expression of CDK1 and the infiltration of CD4 + T cells in NB. Data are presented as mean ± SD. Statistical significance is indicated as follows: *P < 0.05; **P < 0.01; ***P < 0.001.

Article Snippet: A total of 5×10 5 SKNSH and SKNBE cells were cultured in 6-well plates and incubated with DMSO, 10 μM, 15 μM, 20 μM, or 25 μM of the CDK1 inhibitor Ro-3306 (MedChemExpress, HY-12529) for 24 h. Then, the cells were collected, resuspended in binding buffer and stained with Annexin V-FITC/PI (KeyGEN BioTECH, KGA1102) for 15 min at RT in the dark.

Techniques: Expressing, Immunopeptidomics

Journal: Cancer Management and Research

Article Title: Inhibition of CDK1 Promotes Immunogenic Cell Death in Neuroblastoma

doi: 10.2147/CMAR.S569214

Figure Lengend Snippet: The expression of CDK1 negatively correlated with effector cytokine in neuroblastoma. ( A ) The expression of CDK1 was negatively associated with the expression of IL-12A; ( B ) An inverse association was observed between CDK1 and IFNG expression; ( C ) CDK1 expression showed a negative correlation with IRF1 expression; ( D – F ) The correlation between CDK1 and TNF, IL-2, IL-4 did not exhibit statistically significant differences.

Article Snippet: A total of 5×10 5 SKNSH and SKNBE cells were cultured in 6-well plates and incubated with DMSO, 10 μM, 15 μM, 20 μM, or 25 μM of the CDK1 inhibitor Ro-3306 (MedChemExpress, HY-12529) for 24 h. Then, the cells were collected, resuspended in binding buffer and stained with Annexin V-FITC/PI (KeyGEN BioTECH, KGA1102) for 15 min at RT in the dark.

Techniques: Expressing

Journal: Cancer Management and Research

Article Title: Inhibition of CDK1 Promotes Immunogenic Cell Death in Neuroblastoma

doi: 10.2147/CMAR.S569214

Figure Lengend Snippet: Inhibition of CDK1 restrained the growth of neuroblastoma cells. ( A and B ) CDK1 inhibitor induced the apoptosis of SKNBE and SKNSH (n=3); ( C and D ) Inhibition of CDK1 arrested the cell cycle of SKNBE and SKNSH (n=3); ( E and F ) Ro-3306 constricted the migration of SKNBE and SKNSH (n=3); ( G and H ) Ro-3306 constrained the invasion of SKNBE and SKNSH (n=3). Data are presented as mean ± SD. Statistical significance is indicated as follows: *P < 0.05; **P < 0.01; ***P < 0.001.

Article Snippet: A total of 5×10 5 SKNSH and SKNBE cells were cultured in 6-well plates and incubated with DMSO, 10 μM, 15 μM, 20 μM, or 25 μM of the CDK1 inhibitor Ro-3306 (MedChemExpress, HY-12529) for 24 h. Then, the cells were collected, resuspended in binding buffer and stained with Annexin V-FITC/PI (KeyGEN BioTECH, KGA1102) for 15 min at RT in the dark.

Techniques: Inhibition, Migration

Journal: Cancer Management and Research

Article Title: Inhibition of CDK1 Promotes Immunogenic Cell Death in Neuroblastoma

doi: 10.2147/CMAR.S569214

Figure Lengend Snippet: Inhibition of CDK1 promotes the exposure of CRT and HSP70. ( A and B ) Inhibition of CDK1 promotes the exposure of CRT (n=3); ( C and D ) Inhibition of CDK1 facilitates the exposure of HSP70 (n=3). Data are presented as mean ± SD. Statistical significance is indicated as follows: *P < 0.05; **P < 0.01; ***P < 0.001.

Article Snippet: A total of 5×10 5 SKNSH and SKNBE cells were cultured in 6-well plates and incubated with DMSO, 10 μM, 15 μM, 20 μM, or 25 μM of the CDK1 inhibitor Ro-3306 (MedChemExpress, HY-12529) for 24 h. Then, the cells were collected, resuspended in binding buffer and stained with Annexin V-FITC/PI (KeyGEN BioTECH, KGA1102) for 15 min at RT in the dark.

Techniques: Inhibition

Journal: Cancer Management and Research

Article Title: Inhibition of CDK1 Promotes Immunogenic Cell Death in Neuroblastoma

doi: 10.2147/CMAR.S569214

Figure Lengend Snippet: Inhibition of CDK1 promotes the immunogenic cell death of NB. ( A and B ) Inhibition of CDK1 promotes the exposure of CRT detected by immunofluorescence (n=3); ( C and D ) Inhibition of CDK1 facilitates the exposure of HSP70 detected through immunofluorescence (n=3); ( E and F ) Inhibition of CDK1 promotes the secretion of HMGB1 detected by immunofluorescence (n=3). Data are presented as mean ± SD. Statistical significance is indicated as follows: *P < 0.05; **P < 0.01; ***P < 0.001.

Article Snippet: A total of 5×10 5 SKNSH and SKNBE cells were cultured in 6-well plates and incubated with DMSO, 10 μM, 15 μM, 20 μM, or 25 μM of the CDK1 inhibitor Ro-3306 (MedChemExpress, HY-12529) for 24 h. Then, the cells were collected, resuspended in binding buffer and stained with Annexin V-FITC/PI (KeyGEN BioTECH, KGA1102) for 15 min at RT in the dark.

Techniques: Inhibition, Immunofluorescence

Journal: Cancer Management and Research

Article Title: Inhibition of CDK1 Promotes Immunogenic Cell Death in Neuroblastoma

doi: 10.2147/CMAR.S569214

Figure Lengend Snippet: Inhibition of CDK1 restrained the growth of NB cells and enhanced antitumor immune response in vivo. ( A ) photographs of tumors following DMSO or Ro-3306 treatment; ( B and C ) Tumor volume and weight after different treatments (n=6); ( D ) HE and immunohistochemistry staining of tumors following Ro-3306 treatment (n=6); ( E and F ) Representative flow cytometry data showing the proportion of mature dendritic cells (n=6); ( G and H ) Representative flow cytometry data revealing the proportion of M2 macrophages (n=6); ( I – K ) Representative flow cytometry analysis of CD4 + T cells and CD8 + T cells (n=6). ( L and M ) Immunofluorescence staining demonstration the expression levels of CRT and HMGB1 in tumors following Ro-3306 treatment (n=6). Data are presented as mean ± SD. Statistical significance is indicated as follows: *P < 0.05; **P < 0.01; ***P < 0.001.

Article Snippet: A total of 5×10 5 SKNSH and SKNBE cells were cultured in 6-well plates and incubated with DMSO, 10 μM, 15 μM, 20 μM, or 25 μM of the CDK1 inhibitor Ro-3306 (MedChemExpress, HY-12529) for 24 h. Then, the cells were collected, resuspended in binding buffer and stained with Annexin V-FITC/PI (KeyGEN BioTECH, KGA1102) for 15 min at RT in the dark.

Techniques: Inhibition, In Vivo, Immunohistochemistry, Staining, Flow Cytometry, Immunofluorescence, Expressing